Translational_Unit

Part:BBa_K4161004

Designed by: Chang Shen   Group: iGEM22_DKU   (2022-08-19)


Nanobody surface display system based on intimin

Two promotors, Lac promotor, and pTlpA promotor control the expression of the gene. The lac promotor originates from the while the other was added additionally to ensure normal expression in the gut environment. The Neae (BBa_K4161017) protein is the N-terminal fragment of intimin, which can locate itself on the outer membrane of E. coli and display nanobody on the surface of the bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 163
    Illegal PstI site found at 459
    Illegal PstI site found at 707
    Illegal PstI site found at 1154
    Illegal PstI site found at 2397
    Illegal PstI site found at 2465
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 163
    Illegal PstI site found at 459
    Illegal PstI site found at 707
    Illegal PstI site found at 1154
    Illegal PstI site found at 2397
    Illegal PstI site found at 2465
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 163
    Illegal XhoI site found at 2115
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 163
    Illegal PstI site found at 459
    Illegal PstI site found at 707
    Illegal PstI site found at 1154
    Illegal PstI site found at 2397
    Illegal PstI site found at 2465
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 163
    Illegal PstI site found at 459
    Illegal PstI site found at 707
    Illegal PstI site found at 1154
    Illegal PstI site found at 2397
    Illegal PstI site found at 2465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1302


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Categories
Parameters
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